Rat Mitochondrial brown fat uncoupling protein 1 (UCP1) ELISA Kit

Colorimetric

Sandwich ELISA (quantitative)

Rat Mitochondrial brown fat uncoupling protein 1 microplate
Rat Mitochondrial brown fat uncoupling protein 1 standard
Rat Mitochondrial brown fat uncoupling protein 1 detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers

Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gel pack with blue ice.

UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H /OH- are not known. UCPs contain the three homologous protein domains of MACPs. Mitochondrial uncoupling protein 2 is expressed in many tissues, with the greatest expression in skeletal muscle. Although it was originally thought to play a role in nonshivering thermogenesis, obesity and diabetes, it now appears that the main function of UCP2 is the control of mitochondria-derived reactive oxygen species . Chromosomal order is 5'-UCP3-UCP2-3'.

UCP1; SLC25A7; UCP; mitochondrial brown fat uncoupling protein; thermogenin; uncoupling protein 1