Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1) ELISA Kit

P19123

Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Troponin is a central regulatory protein of striated muscle contraction, and together with tropomyosin, is located on the actin filament. Troponin consists of 3 subunits: TnI, which is the inhibitor of actomyosin ATPase; TnT, which contains the binding site for tropomyosin; and TnC, the protein encoded by this gene. The binding of calcium to TnC abolishes the inhibitory action of TnI, thus allowing the interaction of actin with myosin, the hydrolysis of ATP, and the generation of tension. Mutations in this gene are associated with cardiomyopathy dilated type 1Z.Using a human/rodent monochromosomal mapping panel, Song et al. (1996) mapped a human symbolized TNNC1 to chromosome 3 by PCR. Chromosome 3 somatic cell hybrids with various rearrangements were used for finer mapping to 3p21.3-p14.3.

This Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1) ELISA Kit employs a two-site sandwich ELISA to quantitate TNNC1.

Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TNNC1. No significant cross-reactivity or interference between Mouse TNNC1 and analogues was observed.

- Mouse Troponin C, slow skeletal and cardiac muscles microplate
- Mouse Troponin C, slow skeletal and cardiac muscles standard
- Mouse Troponin C, slow skeletal and cardiac muscles detect antibody
- Streptavidin-HRP
- Standard diluent
- Assay buffer
- HRP substrate
- Stop solution
- Wash buffer
- Plate covers

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.

- Do not mix components from different kit lots or use reagents beyond the kit expiration date.
- Allow all reagents to warm to room temperature for at least 30 minutes before opening.
- Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
- Unused wells must be kept desiccated at 4 C in the sealed bag provided.
- Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
- It is recommended that all samples and standards be assayed in duplicate or triplicate.