Mouse Troponin I, fast skeletal muscle (TNNI2) ELISA Kit

P13412

Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

TNNI2 is a fast-twitch skeletal muscle protein, a member of the troponin I gene family, and a component of the troponin complex including troponin T, troponin C and troponin I subunits. The troponin complex, along with tropomyosin, is responsible for the calcium-dependent regulation of striated muscle contraction. Mouse studies show that this component is also present in vascular smooth muscle and may play a role in regulation of smooth muscle function. In addition to muscle tissues, this protein is found in corneal epithelium, cartilage where it is an inhibitor of angiogenesis to inhibit tumor growth and metastasis, and mammary gland where it functions as a co-activator of estrogen receptor-related receptor alpha. This protein also suppresses tumor growth in human ovarian carcinoma.

This Mouse Troponin I, fast skeletal muscle (TNNI2) ELISA Kit employs a two-site sandwich ELISA to quantitate TNNI2.

Mouse Troponin I, fast skeletal muscle (TNNI2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TNNI2. No significant cross-reactivity or interference between Mouse TNNI2 and analogues was observed.

- Mouse Troponin I, fast skeletal muscle microplate
- Mouse Troponin I, fast skeletal muscle standard
- Mouse Troponin I, fast skeletal muscle detect antibody
- Streptavidin-HRP
- Standard diluent
- Assay buffer
- HRP substrate
- Stop solution
- Wash buffer
- Plate covers

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.

- Do not mix components from different kit lots or use reagents beyond the kit expiration date.
- Allow all reagents to warm to room temperature for at least 30 minutes before opening.
- Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
- Unused wells must be kept desiccated at 4 C in the sealed bag provided.
- Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
- It is recommended that all samples and standards be assayed in duplicate or triplicate.