Mouse Troponin T, slow skeletal muscle (TNNT1) ELISA Kit

O88346

Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

TNNT1 is a regulatory complex located on the thin filament of the sarcomere. This complex regulates striated muscle contraction in response to fluctuations in intracellular calcium concentration. This complex is composed of three subunits: troponin C, which binds calcium, troponin T, which binds tropomyosin, and troponin I, which is an inhibitory subunit. This protein is the slow skeletal troponin T subunit. Mutations in this gene cause nemaline myopathy type 5, also known as Amish nemaline myopathy, a neuromuscular disorder characterized by muscle weakness and rod-shaped, or nemaline, inclusions in skeletal muscle fibers which affects infants, resulting in death due to respiratory insufficiency, usually in the second year. Multiple transcript variants encoding different isoforms have been found for this gene.

This Mouse Troponin T, slow skeletal muscle (TNNT1) ELISA Kit employs a two-site sandwich ELISA to quantitate TNNT1.

Mouse Troponin T, slow skeletal muscle (TNNT1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TNNT1. No significant cross-reactivity or interference between Mouse TNNT1 and analogues was observed.

- Mouse Troponin T, slow skeletal muscle microplate
- Mouse Troponin T, slow skeletal muscle standard
- Mouse Troponin T, slow skeletal muscle detect antibody
- Streptavidin-HRP
- Standard diluent
- Assay buffer
- HRP substrate
- Stop solution
- Wash buffer
- Plate covers

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.

- Do not mix components from different kit lots or use reagents beyond the kit expiration date.
- Allow all reagents to warm to room temperature for at least 30 minutes before opening.
- Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
- Unused wells must be kept desiccated at 4 C in the sealed bag provided.
- Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
- It is recommended that all samples and standards be assayed in duplicate or triplicate.