Mouse Tropomyosin alpha-3 chain (TPM3) ELISA Kit

Colorimetric

Sandwich ELISA (quantitative)

Mouse Tropomyosin alpha-3 chain microplate
Mouse Tropomyosin alpha-3 chain standard
Mouse Tropomyosin alpha-3 chain detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers

Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gel pack with blue ice.

Tropomyosin alpha-3 chain is a member of the tropomyosin family of actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosins are dimers of coiled-coil proteins that polymerize end-to-end along the major groove in most actin filaments. They provide stability to the filaments and regulate access of other actin-binding proteins. In muscle cells, they regulate muscle contraction by controlling the binding of myosin heads to the actin filament. Mutations in this gene result in autosomal dominant nemaline myopathy, and oncogenes formed by chromosomal translocations involving this locus are associated with cancer. Multiple transcript variants encoding different isoforms have been found for this gene.

TPM3; RP11-205M9.1; FLJ41118; MGC14582; MGC3261; MGC72094; NEM1; OK/SW-cl.5; TM-5; TM3; TM30; TM30nm; TPMsk3; TRK; hscp30; cytoskeletal tropomyosin TM30; heat-stable cytoskeletal protein 30 kDa; tropomyosin