Mouse Tubulin alpha-4A chain (TUBA4A) ELISA Kit

Colorimetric

Sandwich ELISA (quantitative)

Mouse Tubulin alpha-4A chain microplate
Mouse Tubulin alpha-4A chain standard
Mouse Tubulin alpha-4A chain detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers

Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gel pack with blue ice.

By searching a skeletal muscle EST database, followed by PCR screening of a muscle cDNA library, Stanchi et al. (2000) isolated a cDNA encoding TUBA8. The deduced 449-amino acid protein is 99% identical to the mouse sequence. Quantitative RT-PCR analysis detected TUBA8 expression primarily in heart, skeletal muscle, and testis, with lower expression in brain and pancreas. In mouse embryos, Abdollahi et al. (2009) found wide expression of Tuba8 in developing neural structures. Areas included the cortical preplate, cingulate cortex, hypothalamus, hippocampus, olfactory bulb, and cerebellum. The distribution suggested that Tuba8 has a role in neuronal migration of deep-layer neurons as well as of the cortical plate. Studies of a fusion human TUBA8 protein in cell culture indicated a role in microtubule assembly.

TUBA4A; FLJ30169; H2-ALPHA; TUBA1; tubulin alpha-1 chain; tubulin; alpha 1 (testis specific)