Human Beta-Endorphin (beta-EP) ELISA Kit

Colorimetric

Sandwich ELISA (quantitative)

Human Beta-Endorphin microplate
Human Beta-Endorphin standard
Human Beta-Endorphin detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers

Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gel pack with blue ice.

Beta-endorphin is an endogenous opioid peptide neurotransmitter found in the neurons of both the central and peripheral nervous system.Sequence: "Tyr Gly Gly Phe Met Thr Ser Glu Lys Ser Gln Thr Pro Leu Val Thr Leu Phe Lys Asn Ala Ile Ile Lys Asn Ala Tyr Lys Lys Gly Glu". beta-endorphin is a peptide, 31 amino acids long, resulting from processing of the precursor proopiomelanocortin (POMC). (Note, POMC also gives rise to other peptide hormones, including ACTH , as well alpha- and gamma-MSH, resulting from intracellular processing by internal enzymes known as prohormone convertases.)beta-endorphin is found in neurons of the hypothalamus, as well as the pituitary gland.It is an agonist of the opioid receptors, with evidence suggesting it serves as the endogenous ligand of the u-opioid receptor, the same receptor to which the chemicals extracted from opium, such as morphine and codeine, have their analgesic and addictive effects.