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Human Microtubule-associated proteins 1A/1B light chain 3C (MAP1LC3C) ELISA Kit

22.389,00 €
Zzgl. MwSt.
ArtNr ABK-KTE61740-96Tx50
Hersteller Abbkine Scientific
Menge 96Tx50
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Human (Homo sapiens)
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Lieferbar
Applications notes
This Human Microtubule-associated proteins 1A/1B light chain 3C (MAP1LC3C) ELISA Kit employs a two-site sandwich ELISA to quantitate MAP1LC3C in samples. An antibody specific for MAP1LC3C has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAP1LC3C present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAP1LC3C is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAP1LC3C bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Human Microtubule-associated proteins 1A/1B light chain 3C microplate
Human Microtubule-associated proteins 1A/1B light chain 3C standard
Human Microtubule-associated proteins 1A/1B light chain 3C detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Human Microtubule-associated proteins 1A/1B light chain 3C (MAP1LC3C) ELISA Kit has high sensitivity and excellent specificity for detection of Human MAP1LC3C. No significant cross-reactivity or interference between Human MAP1LC3C and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
Autophagy is a highly regulated bulk degradation process that plays an important role in cellular maintenance and development. MAP1LC3C is 1 of 3 orthologs of the yeast autophagosome protein Atg8. The deduced 147-amino acid protein shares 55% identity with rat Map1lc3. Northern blot analysis detected a 1.9-kb transcript expressed predominantly in placenta, lung, and ovary, with little to no expression in other tissues examined. Western blot analysis of transfected human embryonic kidney cells detected MAP1LC3C proteins of about 16 and 15.5 kD.
MAP1LC3C underwent C-terminal cleavage after the conserved residue gly120. Gly120 was required for posttranslational modification of the MAP1LC3C protein.
Gene ID
440738
Alternative
MAP1LC3C; LC3C; LC3-like protein 2; MAP1 light chain 3-like protein 2
Accession
Q9BXW4

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96Tx50
Lieferbar: In stock
Listenpreis: 22.389,00 €
Preis: 22.389,00 €
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Lieferung vsl. bis 18.09.2025 

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