Human Microtubule-associated serine/threonine-protein kinase 1 (MAST1) ELISA Kit

Colorimetric

Sandwich ELISA (quantitative)

Human Microtubule-associated serine/threonine-protein kinase 1 microplate
Human Microtubule-associated serine/threonine-protein kinase 1 standard
Human Microtubule-associated serine/threonine-protein kinase 1 detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers

Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gel pack with blue ice.

MAST1 shares significant similarity with mouse Mast205 (MAST2). RT-PCR ELISA detected high expression in adult brain, spinal cord, and testis, in fetal brain, and in all specific adult brain regions examined. Expression was moderate in ovary, kidney, and fetal liver, low in pancreas, and very low or undetectable in all other tissues examined.
PCR analysis of rat tissues showed that, like Sast170, Sast124 was expressed exclusively in brain. Immunohistochemical analysis detected strong Sast124 staining in neurons of the subventricular zone and granule cells of the olfactory bulb, islands of Calleja, hippocampal dentate gyrus, and cerebellum. Sast124 localized in nuclei of neurons, in glia-like cell bodies in the corpus callosum, and in fiber bundles in the spinal trigeminal and solitary tracts.

MAST1; KIAA0973; SAST; SAST170; syntrophin associated serine/threonine kinase