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Human A Disintegrin And Metalloprotease 12 (ADAM12) ELISA Kit

23.865,00 €
Zzgl. MwSt.
ArtNr ABK-KTE60910-96Tx50
Hersteller Abbkine Scientific
Menge 96Tx50
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Human (Homo sapiens)
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Lieferbar
Applications notes
This Human A Disintegrin And Metalloprotease 12 (ADAM12) ELISA Kit employs a two-site sandwich ELISA to quantitate ADAM12 in samples. An antibody specific for ADAM12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADAM12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAM12 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAM12 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Human A Disintegrin And Metalloprotease 12 microplate
Human A Disintegrin And Metalloprotease 12 standard
Human A Disintegrin And Metalloprotease 12 detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Human A Disintegrin And Metalloprotease 12 (ADAM12) ELISA Kit has high sensitivity and excellent specificity for detection of Human ADAM12. No significant cross-reactivity or interference between Human ADAM12 and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
ADAM12 encodes a member of the ADAM (a disintegrin and metalloprotease) protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This gene has two alternatively spliced transcripts: a shorter secreted form and a longer membrane-bound form. Similarly to myogenin, a marker of early muscle differentiation, mouse meltrin-alpha is expressed in neonatal muscle and bone, and its expression increases dramatically in response to the induction of differentiation. Immunocytochemical localization and functional expression studies suggested that meltrin-alpha may be involved in myotube formation.
Gene ID
8038
Alternative
ADAM12; RP11-295J3.5; MCMP; MCMPMltna; MLTN; MLTNA; a disintegrin and metalloproteinase domain 12 (meltrin alpha); meltrin alpha
Accession
O43184

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96Tx50
Lieferbar: In stock
Listenpreis: 23.865,00 €
Preis: 23.865,00 €
lieferbar

Lieferung vsl. bis 18.09.2025 

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