Vergleich

Human Abl interactor 1 (ABI1) ELISA Kit

22.389,00 €
Zzgl. MwSt.
ArtNr ABK-KTE60868-96Tx50
Hersteller Abbkine Scientific
Menge 96Tx50
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Human (Homo sapiens)
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Lieferbar
Applications notes
This Human Abl interactor 1 (ABI1) ELISA Kit employs a two-site sandwich ELISA to quantitate ABI1 in samples. An antibody specific for ABI1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyABI1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ABI1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ABI1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Human Abl interactor 1 microplate
Human Abl interactor 1 standard
Human Abl interactor 1 detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Human Abl interactor 1 (ABI1) ELISA Kit has high sensitivity and excellent specificity for detection of Human ABI1. No significant cross-reactivity or interference between Human ABI1 and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
ABI1 has been found to form a complex with EPS8 and SOS1, and is thought to be involved in the transduction of signals from Ras to Rac. In addition, the encoded protein may play a role in the regulation of EGF-induced Erk pathway activation as well as cytoskeletal reorganization and EGFR signaling.
Abi1 is adoptor protein. It interacts with c-Abl and WAVE2 which is actin polymerization regulator. It is known that Abi1 enhances the phosphorylation of WAVE2 by c-Abl. The phosphorylation of c-Abl promotes actin polymerization.Furthermore, Abi1 is component of WAVE complex. Some research paper showed that knock down of Abi1 by si(short interffering)RNA promoted degradation of WAVE complex proteins.
Gene ID
10006
Alternative
ABI1; RP13-16H11.4; ABI-1; E3B1; NAP1BP; SSH3BP; SSH3BP1; Abelson interactor 1; Abl-interactor protein 1 long; eps8 binding protein; interactor protein AblBP4; nap1 binding protein; spectrin SH3 domain binding
Accession
Q8IZP0

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96Tx50
Lieferbar: In stock
Listenpreis: 22.389,00 €
Preis: 22.389,00 €
lieferbar

Lieferung vsl. bis 18.09.2025 

Vergleichen

Auf den Wunschzettel

Angebot anfordern

Lieferzeit anfragen

Technische Frage stellen

Bulk-Anfrage stellen

Fragen zum Produkt?
 
Schließen