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Human Monocarboxylate transporter 4 (SLC16A3) ELISA Kit

22.389,00 €
Zzgl. MwSt.
ArtNr ABK-KTE60625-96Tx50
Hersteller Abbkine Scientific
Menge 96Tx50
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Human (Homo sapiens)
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Lieferbar
Applications notes
This Human Monocarboxylate transporter 4 (SLC16A3) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC16A3 in samples. An antibody specific for SLC16A3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC16A3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC16A3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC16A3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Human Monocarboxylate transporter 4 microplate
Human Monocarboxylate transporter 4 standard
Human Monocarboxylate transporter 4 detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Human Monocarboxylate transporter 4 (SLC16A3) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLC16A3. No significant cross-reactivity or interference between Human SLC16A3 and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
Lactic acid and pyruvate transport across plasma membranes is catalyzed by members of the proton-linked monocarboxylate transporter (MCT) family, which has been designated solute carrier family-16. Each MCT appears to have slightly different substrate and inhibitor specificities and transport kinetics, which are related to the metabolic requirements of the tissues in which it is found. The MCTs, which include MCT1 (SLC16A1) and MCT2 (SLC16A7), are characterized by 12 predicted transmembrane domains. Human MCT4 shares 67% amino acid identity with chicken MCT3 (SLC16A8), 43% identity with human MCT1, 45% identity with human MCT2, 27.1% identity with human MCT5, 38.2% identity with human MCT6, 33.2% identity with human MCT7, and 26.5% identity with human XPCT (SLC16A2).
Gene ID
9123
Alternative
SLC16A3; MCT3; MCT4; MGC138472; MGC138474; monocarboxylate transporter 3; monocarboxylate transporter 4; solute carrier family 16 (monocarboxylic acid transporters); member 3; solute carrier family 16; member 3
Accession
O15427

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96Tx50
Lieferbar: In stock
Listenpreis: 22.389,00 €
Preis: 22.389,00 €
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Lieferung vsl. bis 16.10.2025 

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